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Title غربال باكتري هاي سودوموناس فلورسنت حاوي ژن فنازين-1-كربوكسيليك اسيد به منظور كنترل بيولوژيك بيماري پاخوره گندم
Type Thesis
Keywords wheat
Abstract Wheat take-all fungus is Gaeumannomyces graminis var. tritici of wheat in the country is important pathogens. In order to control this pathogen biology, the study of 22 different strains of Pseudomonas fluorescens strains containing phenazine 1-carboxylic acid synthase gene were used. Tracking gene by primers for genes PCA3b and PCA2a antibiotic synthesis gene and the effect on fungal strains were tested. The ability to produce antimicrobial metabolites such as siderophore production, hydrogen cyanide and antibiotics by bacteria and fungi influence on the growth of bacteria in vitro were investigated. The results showed that the bacterial strains capable of producing hydrogen cyanide, siderophore and an antibiotic were phenazine 1-carboxylic acid in vitro. In greenhouse tests of selected strains ability to control take-all on ten varieties of wheat in a completely randomized design with three replications was used. For this purpose, in a preliminary assessment, the resistance of wheat to wheat take-all fungus was evaluated based on the results, the cultivar was selected as zarin madary. Greenhouse test results showed that the bacterial strains to increase growth and reduce the pathogenicity indices were infected plants and the growth in the take-all fungus or fungus control take-all depending on different wheat varieties and strains of bacteria.
Researchers Fereshteh Bayat (Primary advisor) , Fatemeh Jamali (Primary advisor) , Hamidreza Nooryazdan (Advisor) , ُAhmad qasemei (Advisor)