|
Abstract
|
This study was conducted as a factorial experiment in a completely randomized design with microscopic study and comparison of physiological responses of four imported lemons (Brazil) to salinity stress in in vitro culture, and then cultivar micro-connectivity. The tests were based on the resistant Rufflemon and Volkamerana. In in vitro culture, four plant cultivars were prepared and transferred to the laboratory in MS basic culture medium. The root meristem explants of four cultivars were seeded to glass containing MS medium with different auxin and cytokinin growth regulator treatments such as gibberellin (GA) at concentrations (0 and 2), naphthalene acetic acid. (NAA) at concentrations of (0 and 0.5) and benzyl adenine (BA) at concentrations of (0.5, 1 and 2) mg / L with three replications, performed, and incubated in the storage chamber. Observation of morphological changes (number and length of shoots) were transferred. The highest average shoot length of 3.53 cm and 7 shoots were obtained in ordinary lemon cultivar with medium containing 2 mg / l BA plus 2 mg / l GA. For callus production, combination callus treatments of BA with concentrations of (0.5 and 1) 2,4-D with concentrations of (0.5, 1 and 2) Kineitn with concentrations of (0, 0.5 and 1) mg / L was tested. After investigating the best callus environment, the results showed that conventional lime and lemon cultivars in the medium containing 1 mg / l 2,4-D and 0.5 mg / l Kineitn produced the highest production. They had calluses. Calluses and explants cultured in the best treatment were subjected to salinity stress at different concentrations of 0, 4, 8 and 12 g sodium l-NaCI for three weeks. Storage conditions of all cultures (seed cultivation, reproduction and germination stage, establishment stage and salinity treatment) were carried out in a growth chamber at 25 ± 3 ° C. After stress application, some biochemical parameters such as total protein, proline, and some antioxidants such as peroxidase and
|