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Abstract
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The study aimed at gaining mechanistic insights into the modulation of cisplatin-induced (cDDP, 12 mg/kg-IP) renal toxicity by quercetin, catechin, and genistein in Wistar rats. Blood was analyzed for alterations in acute renal biomarkers (KIM-1, Cystatin-C, GGT, BUN, CR, UA) together with the changes in the activities of antioxidant biomarkers (TAS, TTH, GSH) and cellular damage indicators (MDA, AOPP and 8-OHdG). Additionally, alterations in the activities, variations in genetic expression of antioxidant enzymes (CAT, SOD, GPx, GST, AE, GR) and histomorphological lesions in the kidneys were studied. Quercetin, catechin, and genistein restored the renal antioxidant status and thiol levels, highlighting their potential in conferring protection against cDDP-induced damage to renal parenchyma as evidenced by the reduction of lesion load in the affected kidneys. Results demonstrated their capabilities to modulate the activities of GCLC, GCLM, effector thiols, and other components of antioxidant machinery to offset cDDP-induced nephrotoxicity. Furthermore, it can be gleaned from our data that quercetin proved to be more effective in alleviating cDDP-induced kidney damage in comparison to catechin and genistein.
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