Amir Vazirizadeh

Background: As a major public health issue, the skin cancer is a leading reason of death and has resulted in significant financial and human losses globally. Numerous environmental and internal variables may both drive and exacerbate the pathophysiology of the skin cancer. Marine herbs and animals, including marine sponges, cucumbers, and squirts, have been shown to have cytotoxic consequences on cancerous cells in prior research. Methods: In this study, the mitochondria isolated from melanoma cells via differential centrifugation and treated with various concentrations (1250, 2500 and 5000 µg/ml) of methanolic extract of C. tubulosa. Then MTT, ROS, MMP decline, mitochondrial swelling, cytochrome c release and flow cytometry assays were performed on them. Results: The results of MTT assay showed that the IC50 of C. tubulosa extract is 2500 μg / ml and C. tubulosa extract induced a selectively significant (P<0.05) concentration-dependent decrease in the SDH activity in cancerous skin mitochondria. The ROS results also showed that all concentrations of C. tubulosa extracts significantly increased ROS production, MMP decline and the release of cytochrome c in cancer groups’ mitochondria. The swelling of mitochondria isolated from the cancer group was significantly increased compared to the control group. In addition, the results of apoptosis assay showed that addition of root extract of Cistanche tubulosa on melanoma cells increased apoptosis, while it had no effect on control non tumor cells. Conclusion: Based on these results, the presence of potentially bioactive compounds in C. tubulosa make this Persian Gulf coastal herb a strong candidate for further molecular studies and clinical research in the field of melanoma cancer therapy