03 مهر 1400

شهریار عصفوری

مرتبه علمی: دانشیار
نشانی: دانشکده مهندسی نفت، گاز و پتروشیمی - گروه مهندسی شیمی
تحصیلات: دکترای تخصصی / مهندسی شیمی
تلفن: 88019360
دانشکده: دانشکده مهندسی نفت، گاز و پتروشیمی

مشخصات پژوهش

عنوان Investigations of antioxidant potential and protective effect of Acanthophora algae on DNA damage: An electrochemical approach
نوع پژوهش مقالات در نشریات
کلیدواژه‌ها
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مجله MICROCHEMICAL JOURNAL
شناسه DOI
پژوهشگران ائمه باقری حشکوایی (نفر اول) ، صدیقه هاشم نیا (نفر دوم) ، شهریار عصفوری (نفر سوم)

چکیده

In this paper, the electrochemical method was used as a rapid and sensitive method to measure the antioxidant activity of Acanthophora red macro-algae (collected from the Persian Gulf coast of Bushehr-Iran). The algae extract was obtained by a combination of maceration and microwave methods, and then the important volatile and bioactive compounds were identified by GC-Mass analysis. To prepare the sensor and improve the electrochemical signal, gold nanoparticles synthesized by the green method were used to modify the electrode surface. Differential pulse voltammetry method (DPV) was used to investigate the inhibitory effect of the extract on ABTS and DPPH radicals. To investigate the ability of the Acanthophora algae extract to prevent DNA damage induced by Fenton reaction, an electrochemical biosensor was designed based on the immobilization of human interleukin-2 (IL-2) gene probe on the surface of gold nanoparticle-modified carbon screen-printed electrode. Then, the protective effect of the algae extract was investigated by electrochemical impedance spectroscopy. Finally, the toxicity effect of Acanthophora algae extract on the HT-29, SKOV3, MCF-7, and SKMES-1 cancer cell lines was evaluated by MTT assay.