حمیدرضا نوریزدان

Due to the prohibition use of chemical materials such as malachite green and antifungal compounds, it is necessary to find a promising compound without effect on minimal and environment. Garlic (Allium sativum) is one of the plants that its antifungal and antimicrobial properties have been studied and indicate the appropriate possibility of replacing it with chemical destructive drugs. Effects of the aqueous, ethanol and methanol extracts of garlic on Saprolegnia, determination of minimum inhibitory concentration of fungus growth and comparison of the inhibitory effects of different concentrations of garlic extract with Malachite green and Bronopol, was the purpose of this study. In this study, Saprolegnia fungus were isolated, identified and purified from the infected samples. After preparation of the extracts, the amount of 100, 50, 5, 3, 0.5 and 0.3 µl of extract were added to each Petri using wells method. MIC for aqueous, ethanol and methanol extract were 0.3 mg/ml. MIC of Malachite green and Bronopol were 0.15 and 10 mg/ml. The highest growth of fungus were observed in 3 mg/ml concentration of ethanol extract of garlic. Garlic extract has anti-fungal properties and due to herbal and no side effects to aquatic and users, can be used as an antifungal in the aquaculture industry.