December 21, 2024

Mehdi Mohammadi

Academic Rank: Assistant professor
Address:
Degree: Ph.D in Biotechnology
Phone: 0772150668
Faculty: Persian Gulf Research Institue

Research

Title
Identification and design of microsatellite marker primers in common elephantfish (Eleutheronema tetradactylum, Shaw, 1804) Persian Gulf
Type Thesis
Keywords
ريزماهواره، ماهي راشگوي معمولي، (Eleutheronema tetradactylum, خليج فارس
Researchers Mehdi Mohammadi (Primary advisor) , Seyed Javad Hosseini (Advisor)

Abstract

Biodiversity is a critical part of natural capital. Many of our needs are met by this capital. Biodiversity is a type of nature insurance against bad accidents. The importance of fully understanding the fish for the optimal use of these reserves is not hidden from anyone aware of fisheries issues. Today, with molecular technologies, differences between races, genotypes, or individuals can be carefully examined in population genetic research. Identification, conservation, and sustainable use of genetic diversity are essential to the success of any breed or biotechnology program. Microsatellites have been found in the DNA of the nucleus of all eukaryotes and even some prokaryotes. Microsatellites are the shortest consecutive arrangements (1 to 6 pairs of games) in which the number of repeating units in each location varies from a few bases to about 30 bases. One of the major problems of microsatellites is the need to create and screen DNA gene libraries for microsatellite regions. This requires recognizing the existing DNA sequences under study, which is very time consuming and costly. Common elephantfish (Eleutheronema tetradactylum) has long been of special interest to beachgoers and is in the group of privileged fish in the Persian Gulf and the Sea of ​​Oman. This species has the highest price among the aquatic animals of the Persian Gulf after Pampus argenteus white halva. In this study, 79 samples were taken from Khuzestan provinces (Choobdeh Abadan, Mahshahr and Hindijan ports) and Bushehr (Jalali port of Bushehr and Bandar Dir) using fishing boats equipped with turbochargers. The size of the sequenced plots was 982-662 bp. Six microsatellite allele sites were examined. Five stations were complete (pure) and one station was composite. 3 pairs were polymorphic and 3 pairs were monomorphic. The highest number of alleles observed in Eletet2 (AB697177) and related to the samples of Bushehr province (5 alleles) and the lowest value in Eletet16.0 (AB697180) and relate