Phytohormone-induced changes in growth, physiology, and biochemistry of Aurantiochytrium sp. for sustainable bioproduction, By Mehdi Mohammadi

Research

Title	Phytohormone-induced changes in growth, physiology, and biochemistry of Aurantiochytrium sp. for sustainable bioproduction
Type	Article
Keywords	Aquatic ecosystemsBioactive compoundsFatty acidsMicroalgaeGene expressionOxidative stress response
Journal	BIORESOURCE TECHNOLOGY
DOI	https://doi.org/10.1016/j.biortech.2024.131249
Researchers	Zahra Shafagat (First researcher) , Farzaneh Najafi (Second researcher) , Ramezan Ali Khavari Nejad (Third researcher) , Mehdi Mohammadi (Fourth researcher) , Sataar Tahmasebi (Fifth researcher)

Abstract

اThe study aimed to assess the effects of nine combinations of phytohormones, salicylic acid (SA), gibberellic acid (GA), and jasmonic acid (JA) on the growth, physiology, and biochemistry of Aurantiochytrium sp. Parameters like optical density (OD), biomass, protein content, hydrogen peroxide (H2O2), malondialdehyde (MDA), catalase activity (CAT), and gene expression (malic enzyme (ME) and acetyl-CoA carboxylase (ACCase)) were assessed at various cultivation stages (24, 48, 72, and 96 h). The research also analyzed fatty acid composition, unsaturated fatty acids (UFA), saturated fatty acids (SFA), and the UFA to SFA ratio (USS) to understand the biochemical changes induced by phytohormones. Results demonstrated that modifying phytohormone concentrations significantly affected the characteristics of the microalgae, particularly in correlation with different growth stages, emphasizing the necessity of precise control of phytohormone levels for optimizing cultivation conditions and enhancing bioactive compound production in Aurantiochytrium sp.