Introduction & Objective: Drug resistance is one of the main causes of failure in chemotherapy treatment of various types of cancer, including pancreatic cancer. Arsenic trioxide is known to be an effective compound on pancreatic cancer cells. Also, the combination of arsenic trioxide with other compounds reduces drug resistance and has a great anti-tumor effect in pancreatic cancer. On the other hand, copper oxide nanoparticles have a toxic effect on cancer cells. In this study, we investigated the effect of arsenic trioxide and nanoparticles and the simultaneous effect of these two compounds on pancreatic cancer cells with the aim of reducing the expression of genes involved in drug resistance. Materials and Methods: The effect of arsenic trioxide and copper oxide nanoparticles on bioavailability, cell migration and colonization power in PANC-1 cell line was evaluated. The cell cycle and apoptosis were examined by flow cytometry at 48 μM of arsenic trioxide and 45 μg / ml nanoparticles at 48 h. Altered expression of drug resistance genes in treated and control cells was measured by real time PCR.
Results: The results of this study showed that arsenic trioxide at a concentration of 20 μM and copper oxide nanoparticles lead to bioavailability, cell migration and colonization power in PANC-1 cell line. It was also observed that arsenic trioxide at a concentration of 20 μM in 48 hours was observed that arsenic trioxide and copper oxide (I) nanoparticles also reduced the cell population of phase G1 and stopped in phase S, the percentage of cells Apoptosis of cells treated with arsenic trioxide and copper oxide nanoparticles indicates the significant effect of them inducing apoptosis. expression lncRNA HOTAIR and HOTTIP decreased under the influence of a combination of copper oxide nanoparticles and arsenic trioxide.
Discussion & Conclusion: Since PANC-1 cell line is resistant to arsenic, in order to prevent it at high concentrations and reduce side effects on patients,