Introduction: Every year, millions of people around the world suffer from burns and
accidents, and thousands of people die due to severe skin injuries and delays in repairing
damaged tissue and preventing bleeding. Studies have shown that the bioactive compounds of
the algea such as polysaccharides and their fatty acids have biological, chemical and physical
properties along with biocompatibility and biodegradability that make them suitable for
various applications such as wound healing.The current study aims to investigate the effects
of two groups of compounds (fatty acids and polysaccharides) in the microalgae Cyanothece
sp. isolated from the coast of Chabahar was performed on the wound healing caused by rat
skin burns.
Materials and methods: Culture of isolated algae and extraction of fatty acids and
polysaccharides from microalgae Cyanothece sp. done. The experimental ointment was
prepared using the extracted compounds in the amount of 5% with oserin base. 40 Wistar
albino male rats were divided into four groups: positive control (Bio-oil ointment), negative
control (Oserin), treated with algal fat, and treated with algal polysaccharide ointment. A
burn wound with dimensions of one square centimeter was created on the back of the mouse.
Treatment with ointment was done as follows: in the first week, twice a day, in the second
week, once every day, in the third week, once every two days, and in the fourth week, no
treatment. Regular measurement and imaging of the wound, sampling and microscopic slide
preparation of the tissue for histopathological examination and blood sampling to examine
the repair and inflammatory factors (cytokines IL-6β, IL-1 and TNFa, CRP protein) were
performed.
Results: The external examination of the wounds showed that on the 14th day, the negative
control group had inflamed and necrotic tissue and the extent of the wound was greater than
that of the treatment groups. On the 21st day, the skin tissue was fully healed in all groups,
but t