Objective: Despite advances in treatment and overall reduction in coronary artery disease-related mortality, it remains the leading cause of death in both men and women. A group of coronary artery disease sufferers are diagnosed with this disease at a younger age than others; In this case, this disease is called premature coronary artery disease. Premature coronary artery disease increases the risk of major cardiac events (MACE). Major cardiac events are a clinical endpoint that is often used in cardiovascular research. Considering that the occurrence of major cardiac events imposes a high cost on the society and the individual, it is important to identify its molecular mechanism.One of the methods of studying the molecular mechanism of MACE is to determine the genotype. Premature coronary artery disease is a multi factorial and multi genetic disease that is the result of the interaction of genetic predisposition and environmental factors. Considering the role of chromosomal region 9p2.1 in increasing the risk of cardiovascular disease, the aim of the present study is to investigate and determine the genotype of SNP rs4977574 in people with premature coronary artery disease among a group of these patients who have MACE in comparison with the group that does not develop MACE.
Materials and Methods: In this nested case-control study, peripheral blood samples from 136 young patients with premature coronary artery disease (men less than 45 years old and women less than 55 years old) among a group of patients with coronary artery occlusion included in the Coronary Atherosclerosis Cohort (THC-PAC) participated in Tehran Heart Center and were monitored for the occurrence of MACE during 64 months.DNA extraction from white blood cells was done using phenol chloroform method. Genotyping of rs4977574 polymorphism in patients was done by ARMS PCR method. Data analysis and relationship between rs4977574 polymorphism genotypes and MACE was done using SPSS software and p-value les