In the synthesis of nanoparticles in physical and chemical methods, high temperature and high operational pressure is required, and besides, there are other disadvantages such as pollution due to the use of various chemical materials, costly, low efficiency and gross products. Today, the use of biological methods to produce nanoparticles with minimum pollution for the environment and human is considered. in this method , which is known as green chemistry or biological synthesis , species of living organisms such as bacteria , fungi , plants , algae , are used for the synthesis of nanoparticles , in which algae , because of the abundance , diversity and existence of effective secondary metabolites , have many important at the adsorption of nanoparticles . In the biological synthesis of nanoparticles, natural ingredients in plant extracts are harmful to chemical compounds such as enzymes, carbohydrates and terpenoid. in this study , the water extract of three species of brown algae was used as selective and pure biosynthesis, in this method Sargassum latifolium brown algae, Cystoseira myrica and Padina australis hauck were used in the presence of solution containing copper (II) sulfate, which resulted in the production of copper (I) oxide nanoparticles in red , orange and yellow . In order to verify the synthesis of nanoparticles and to investigate their properties and characteristics, X-ray diffraction (XRD) was used with scanning electron microscopy (SEM) and scanning electron microscopy (SEM), which confirmed the synthesis of copper (I) oxide nanoparticles in the size of 10 to 30 nm in spherical shapes and high purity. The synthesized nanoparticles were then selected in the presence of sargassum algae and cystoseira due to high purity to study the anti - bacterial effect. in this study , the effect of nanoparticles on positive warm bacteria (Staphylococcus aureus), MBC bacteria (Pseudomonas aureus) and MIC bacteria (E. coli) sho