chitin is one of the main components of shrimp shell, which is one of the most abundant natural renewable biological resources and has many applications in biomedicine, agriculture, water purification and cosmetics. In addition, it can be easily recycled. Waste processing and extraction of the valuable chitin compound from shrimp skin will prevent environmental pollution in addition to economic benefits. In recent years, efforts have been made to use green and environmentally friendly solvents as an alternative to traditional solvents and chemicals for chitin extraction, and this effort is still ongoing.
The aim of this research is to optimize the conditions for extracting acetylated chitin with high purity from shrimp waste with the help of deep eutectic solvents based on amino acids.
In this thesis, asparagine-lactic acid (8:1) deep eutectic solvent was used to extract O-acetylated chitin and the effect of parameters such as temperature, time, solvent-sample weight percentage and water-solvent weight percentage were investigated and in Each experiment measured the amount of protein, chitin, and the degree of acylation of the extracted chitin.
In this study, under optimized conditions; The temperature of 140 degrees Celsius, the duration of 10 hours, the ratio of the used deep eutectic solvent to the sample (40:1) and without using water as an auxiliary solvent, O-acetylated chitin was extracted with a purity of 98.1% and acylation degree of 0.63. Chitin extracted under optimal conditions was measured by XRD, SEM, TGA analysis
The results obtained from this research showed that deep eutectic solvents based on amino acids can be suitable candidates for extracting chitin and other useful compounds from marine and terrestrial organisms. In this study, the mixture of asparagine and lactic acid with a molar ratio of 1 to 8, as a deep eutectic solvent, performed the three simultaneous roles of calcium carbonate removal, protein removal, and chitin acylation. The experime