This study was aimed to survey toxicity of waterpipe wastes leachates on Peronia peronii
in aquatic and sediment environments as two exposure media. For this, leachates of four tobacco types
including burnt traditional tobacco (BTT), fresh traditional tobacco (FTT), burnt fruit-flavored tobacco
(BFT) and fresh fruit-flavored tobacco (FFT)) were prepared and used to assess their toxic effects on P.
peronei in two aquatic and sediment media. The invivo toxic effects of five different concentrations of
waterpipe tobacco waste leachates on P. peronii were evaluated. The LC 50 values of BTTs leachates to P.
peronii were 17.50, 16.05, 11.31 and 9.38 g/L at exposure times of 24, 48, 72 and 96 h, respectively in
aquatic media. These values for BFTs leachates were 14.86, 12.38, 9.53 and 7.46 g/L at exposure times of
24, 48, 72 and 96 h, respectively. In the case of sediment media, the LC50 values of BTTs leachates were
15.33, 13.70, 9.09 and 6.70 g/L at exposure times of 24, 48, 72 and 96 h, respectively while these values for BFTs leachates were 12.00, 10.32, 8.20 and 5.65 g/L. Fruit-flavored tobacco leachates had significantly higher toxicity than traditional tobacco leachates for P. peronii. The findings also showed significant differences between the LC
50 values of different leachates in different media of water and sediment.The results demonstrated that even small amount of tobacco waste (~5 to 6 g/L) can lead to P. peronii mortality and may also pose a hazard to other aquatic and benthic organisms. The results obtained from the present study can be used as a baseline data to assess local effects causing from unsafe disposal of postconsumption tobacco waste in beach areas. In addition, these findings can lead to encouraging decisionmakers to focus more on the types of tobacco waste in the municipal solid waste management system and
to implement a source separation process for these wastes.