Background: Resistance training along with the consumption of protein supplements increases protein synthesis and then causes hypertrophy. One of the hypertrophy pathways in the kidney can be the signaling pathway (Wnt/ GSK-3β/ TSC2/ S6K). during which mTOR is activated in the kidney. Its activation causes kidney disease and disease progression. Aim: According to the aim of this research, the effect of eight weeks of resistance training and spirulina supplementation for 8 weeks and its effect on the signaling pathway (Wnt/GSK-3β/TSC2/S6K) in all male rats.
Methodology: In this present research, male Sprague Dawley rats were formed in the form of experimental design and statistical population of this research. A number of 32 young male rats with 3 months of age and an average weight of 150±20 grams were randomly prepared and during the research period, each rat was kept in a separate cage made of transparent polycarbonate and in an environment with a temperature of 22±2 degrees Celsius. degree, humidity 55±4% and light-dark cycle 12:12 hours were maintained. After the familiarization phase, the rats were randomly divided into four groups: control, resistance exercise, spirulina supplement, and exercise-supplement. Spirulina in the amount of 200 mg/kg/day was added to the water of the supplemented and exercise-supplemented groups and continued until the end of the eighth week. The resistance training protocol consisted of eight weeks of climbing a 1 meter high ladder. 24 hours after the last training session, the rats were dissected and the isolated tissue was kept at -70 temperature, then the expression level of the dependent variables of the research was measured using the REAL TIME-PCR method. Cross-sectional analysis of variance was used to analyze the data at a significance level of 0.05.
Result: Wnt gene expression in spirulina group (P = .306), resistance training (P = .133) and the interaction of resistance training and spirulina (P = .480) were not significan