Persian Gulf β-chitin was extracted from the cuttlebone of Sepia Pharaonis and chitosan was prepared through deacetylation using microwave technique. β- Chitin and β-chitosan were characterized for their structural and physical (CHN, DDA, FT-IR, NMR, XRD and Viscometric analysis) properties. The purity, DDA and molecular weight of present chitosan were found 85.3%, 90% and 350.06 kDa. Further, acyl chitosan derivatives were prepared by the reaction of β-chitosan with several derivatives of acyl chlorides.