This study investigated the effect of the dietary supplementation of bovine lactoferrin (LF) on growth performance, hematological and immunological parameters, antioxidant enzymes activity and disease resistance against Vibrio harveyi in yellowfin sea bream (Acanthopagrus latus) fingerling. The fish with initial body weight 10?±?0.3?g were randomly distributed at 10 fish per each 250?L fiberglass tank, and fed with four experimental diets (a control basal diet and three supplemented diets with 400, 800 and 1200?mg?LF kg?1 diet) for 8 weeks. The obtained results showed that fish fed with LF supplemented diets had significantly higher final body weight as compared to control diet (P?0.05). There were no significant differences between LF-treatments and the control group in white blood cell counts, red blood cell counts, hemoglobin and hematocrit. Total protein and complement activity (ACH50) in the serum of yellowfin sea bream were enhanced with increasing the dietary LF supplementation level (P?0.05). The mucus lysozyme activity in fish fed on 800 and 1200?mg LF kg?1 was significantly higher than those fed on 400?mg LF kg?1 and control fish (P?0.05). None of the antioxidant enzymes (catalase, glutathione reductase, glutathione S-transferase) was affected by LF supplementation (P?>?0.05). Fish fed with dietary LF had a significantly higher survival rate than those fed with the control diet after challenge with Vibrio harveyi (P?0.05). These results revealed that diet supplementation in A. latus especially with 1200?mg LF kg?1 improve fish growth performance and immune parameters, as well as survival rate against Vibrio harveyi.