The study investigated the effects of embryonic thermal manipulation (TM) and post-hatch guanidinoacetic acid (GAA) supplementation on male broiler chickens exposed to chronic heat stress (HS). Ross 308 eggs (n = 710) were randomly assigned to control (37.8°C, 56% RH) or TM (39.5°C, 65% RH for 12 hours/day from embryonic day 7 to 16) treatments. After hatching, chicks were further assigned to four dietary treatments (n = 12 birds/pen, 5 replicates/treatment): control, control with 1.2 g/kg GAA supplementation (CS), TM, and TM with 1.2 g/kg GAA supplementation (TMS). All birds were subjected to chronic HS (32-36°C, 6 hours/day, 55% RH) from day 28 to 42. Embryonic TM treatment decreased hatchability, hatching weight (HW), and facial temperature (FT). During the pre-HS period (days 1-28), no significant differences in feed conversion ratio (FCR) and mortality were observed, although the TM group exhibited the lowest body weight gain (BWG). Following HS exposure (days 29-42), the TMS group displayed significantly higher BWG than the control and CS groups. The TM and TMS groups also demonstrated significantly lower FCR and mortality rates during this period. Across the entire period (days 1-42), BWG was significantly higher in the TMS group compared to other groups. Furthermore, TM and TMS treatments were associated with lower mortality rates, improved FCR, better European Performance Efficiency Index (EPEI), and reduced abdominal fat deposition. The experimental treatments did not significantly affect intestinal morphology or most blood parameters, except triiodothyronine (T3), thyroxine (T4), and uric acid. Plasma concentrations of T3, T4, and uric acid were significantly lower in the TM and TMS groups compared to the control and CS treatments. The findings suggest that a combined strategy of embryonic TM and post-hatch dietary GAA supplementation may not only alleviate the detrimental effects of HS but also promote beneficial physiological responses in broiler chicke