11 آذر 1403
ابراهيم ستوده

ابراهیم ستوده

مرتبه علمی: دانشیار
نشانی: دانشکده علوم و فناوری نانو و زیستی - گروه علوم شیلاتی
تحصیلات: دکترای تخصصی / مهندسی منابع طبیعی
تلفن: 09173740528
دانشکده: دانشکده علوم و فناوری نانو و زیستی

مشخصات پژوهش

عنوان Effect of freezing rate for cryopreservation of Persian sturgeon (Acipenser persicus) spermatozoa
نوع پژوهش مقالات در نشریات
کلیدواژه‌ها
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مجله THERIOGENOLOGY
شناسه DOI 10.1016/j.theriogenology.2015.10.018
پژوهشگران Mohammad Sadegh Aramli (نفر اول) ، Karim Golshahi (نفر دوم) ، Rajab Mohammad Nazari (نفر سوم) ، ابراهیم ستوده (نفر چهارم) ، Salim Aramli (نفر پنجم) ، Ensieh Habibi (نفر ششم به بعد)

چکیده

This study examined the effect of freezing rate (?10 °C, ?15 °C, ?20 °C, ?30 °C, and ?40 °C/min) on motility parameters, rates of fertilization and hatching, ATP content, and indices of oxidative stress including thiobarbituric acid reactive substances and carbonyl derivatives of proteins in Persian sturgeon (Acipenser persicus) sperm. After sampling, sperm was diluted in an extender composed of 23.4-mM sucrose, 0.25-mM KCl, and 30-mM Tris-HCl, pH 8.0, containing 10% methanol and subsequently frozen in a programmable freezer. For postthaw sperm that were frozen at a rate of ?40 °C/min, sperm motile duration (134 ± 27.01 seconds), sperm motile percent (60 ± 4.1%), fertilizability (72 ± 8.36% for fertilization rate and 65 ± 7.58% for hatching rate), and ATP content (4.8 ± 0.57 nmol/108 sperm) were significantly higher than for sperm frozen at any of the four slower rates (P < 0.05). Moreover, sperm cryopreserved using the fastest freezing rate had significantly lower levels of thiobarbituric acid reactive substances (0.5 ± 0.05 nmol/108 sperm) and carbonyl derivatives of proteins (41.3 ± 4.9 nmol/108 sperm) than sperm cryopreserved using all other freezing rates (P < 0.05). In addition, there is a significant difference (P < 0.05) between fresh sperm and the recovery of cryopreserved Persian sturgeon sperm using programmable freezing with ?40 °C/min being the optimal freezing rate among those tested.