11 آذر 1403
ابراهيم ستوده

ابراهیم ستوده

مرتبه علمی: دانشیار
نشانی: دانشکده علوم و فناوری نانو و زیستی - گروه علوم شیلاتی
تحصیلات: دکترای تخصصی / مهندسی منابع طبیعی
تلفن: 09173740528
دانشکده: دانشکده علوم و فناوری نانو و زیستی

مشخصات پژوهش

عنوان Reliable collection of Caspian brown trout (Salmo trutta caspius) sperm using a catheter
نوع پژوهش مقالات در نشریات
کلیدواژه‌ها
ثبت نشده‌است!
مجله REPRODUCTION IN DOMESTIC ANIMALS
شناسه DOI
پژوهشگران محمد صادق آراملی (نفر اول) ، کریم گلشاهی (نفر دوم) ، اشکان بنان (نفر سوم) ، ابراهیم ستوده (نفر چهارم)

چکیده

The traditional stripping procedure for collecting fish semen is associated with the risk of urine contamination, which may significantly affect semen quality and quantity. The use of a catheter as an alternative method for semen collection may overcome this problem. Therefore, this study compared Caspian brown trout (Salmo trutta caspius) semen parameters (i.e. sperm density, seminal plasma osmolality, motility parameters of spermatozoa analysed using computer-assisted sperm analysis and fertility) between the traditional stripping method and the use of a catheter. All parameter values of the semen collected with a catheter were significantly higher (p < .05; density = 7.67 ± 1.02 × 109 ml?1 and osmolality = 279.28 ± 32.84 mOsm kg?1) than those collected with stripping method (density = 4.85 ± 0.47 × 109 ml?1 and osmolality = 216.42 ± 20.75 mOsm kg?1). Semen collected with a catheter was characterized by higher spermatozoa motility compared with sperm collected via stripping. Similarly, the fertilization ability of sperm collected with a catheter was significantly greater (p < .05) than sperm collected with the traditional stripping method. In conclusion, collection of sperm with a catheter was shown to effectively reduce urine contamination and is therefore recommended for the collection of Caspian brown trout sperm.