22 آذر 1403
فرشته بيات شاه پرست

فرشته بیات شاه پرست

مرتبه علمی: استادیار
نشانی: دانشکده مهندسی کشاورزی - گروه مهندسی تولید و ژنتیک گیاهی
تحصیلات: دکترای تخصصی / اصلاح نباتات
تلفن: 07733445182
دانشکده: دانشکده مهندسی کشاورزی

مشخصات پژوهش

عنوان
بررسی کشت درون شیشه ای و اندام زایی غیر مستقیم گیاه دارویی گل راعی Hypericum perforatum L
نوع پژوهش مقالات در همایش ها
کلیدواژه‌ها
Hypericum, tissue culture, callus
پژوهشگران زینب پارسامنش (نفر اول) ، فرشته بیات شاه پرست (نفر دوم) ، محمد هدایت (نفر سوم)

چکیده

St Johns Wort (Hypericum perforatum L.) from Hypericaceae family is an important medicinal plant, which its secondary metabolites, hypericin and hyperforine, have several medicinal effects such as antidepressant, antiviral, antibacterial and etc. In this study callus induction of two explants of St. Johns Wort including leaves and shoots were evaluated at different level of growth regulators (zero, 2 and 4 mg/l BA, and 0.1 and 2 mg/l kinetin plus 0.1, 0.2 and 0.3 mg/l 2,4-D) in MS. To evaluate shooting zero, 0.5 and 1 mg/l BA and zero, 0.5 and 1 mg/l kinetin were used. In order to produce root, growth regulator IBA at concentrations of zero, 0.2, 0.4, 0.6, 0.8 and 1 mg/l were used. After 4 weeks at 21°C and 16 hours light, the callus induction, shooting and rooting were measured. Data analysis indicated that the highest patch level of 5% in the number of leaf explants achieved at 0.3 mg/l of 2,4-D. Highest mass of callus observed at single node explants in media containing 2 mg/l BA with 0.1 mg/l of 2,4-D, respectively.