Elicitation of gentiopicroside in Gentiana kurroo Royle shoot cultures with endophytic fungus, By gholamreza Abdi

Research

Title	Elicitation of gentiopicroside in Gentiana kurroo Royle shoot cultures with endophytic fungus
Type	Article
Keywords	fungal elicitors; endophyte; Gentiana kurroo; gentiopicroside; in vitro shoots
Journal	EGYPTIAN JOURNAL OF BOTANY
DOI	10.21608/ejbo.2024.240513.2582
Researchers	Mariadoss Alphonse (First researcher) , Kalaivani Thiagarajan (Second researcher) , Navapara Radha (Third researcher) , Richa Doshi (Fourth researcher) , Nidhi Sabade (Fifth researcher) , gholamreza Abdi (Not in first six researchers) , , Sudhir Singh (Not in first six researchers) , Devanand P. Fulzele (Not in first six researchers) , , Michael Pillay (Not in first six researchers) , Siva Ramamoorthy (Not in first six researchers) , Rajasekaran Chandrasekaran (Not in first six researchers)

Abstract

Gentiopicroside (GPD) is a major secoiridoid glucoside in the herbs of the Gentianaceae family, predominantly in the genus Gentiana. Gentiana kurroo Royle, a native of the Indian Himalayas is rich in GPD in the roots and shoots. It is used extensively for treating various diseases in Ayurvedic medicine. This herb grows above 1600–3500 Masl and is endemic to the Himalayan region and extensively harvested from wild sources for its immense medicinal properties. In this study, in vitro shoot cultures of G. kurroo were stimulated with Trichoderma viride, Aspergillus niger, Fusarium oxysporum, and an endophytic fungal cell wall extract for enhanced production of GPD. The results showed that treatment with the endophytic fungal extract produced a much higher concentration of GPD (30.4 mg/g DW) after 48 hrs of treatment compared with other treatments. The endophytic fungus was identified as Parengyodontium album. This study provides a simple and efficient method for in vitro elicitation of GPD.