Hamid Karamikabir

Objective: Coronary heart disease (CAD) is a multi-factorial and multi-situational disease in which the coronary wall is narrow and blood supply to the heart muscle is limited due to the formation of atherosclerosis plaques. CAD and its symptoms include heart attacks. They are one of the most important causes of death in the world. It is believed that several genes and loci play a role in increasing the probability of contracting this disease. Among these polymorphisms, rs1333049 is associated with CAD disease. The aim of this study is to investigate the association of this polymorphism with CAD in the population of Bushehr province. Materials and Methods: In this study, blood samples of 135 patients with CAD and 59 healthy controls were collected from the heart hospital of Bushehr. DNA extraction from white blood cells was done using chloroform method. Genotyping of rs1333049 polymorphism in patients was done by ARMS PCR method. Data analysis and relationship between rs1333049 polymorphism geno types and CAD were done using SPSS software and p-value less than 0.05 (Exact sig˂0.05) was considered as significant level. Results: In this study, Exact sig=0.223 was obtained for genotype, and this value was also calculated for gender, blood pressure, and smoking, which respectively obtained Exact sig=0.001, Exact sig=0.02, and Exact sig=0.001. Came. Conclusion: Although the type of genotype is not related to the occurrence of major cardiac events in patients with coronary artery occlusion, CAD shows a highly significant relationship with smoking, blood pressure, and male gender.