Background: Stomach cancer is a fatal disease that is reported as the fifth most common cancer
and the fourth cause of death worldwide in 2020. The limited effectiveness and side effects of current
treatments, the resistance of stomach cancer cells to drugs and chemotherapy have caused new
treatment strategies, especially the use of nanotechnology and nanoparticles, to be considered.
Among the anti-tumor nanotherapies, one of the effective strategies is the use of magnetic
nanoparticles to improve targeting, gene silencing and drug delivery, which provides a positive and
powerful performance to solve the problems of conventional cancer chemotherapy. For this purpose,
in the current research, the magnetic nanodrug delivery system, including two therapeutic
components attached to cobalt ferrite nanoparticle and its polymer coating, has been selected
Aim: In this study, the use of cobalt ferrite magnetic nanoparticles (CoFe2O4) as targeted carriers
in the controlled delivery of cisplatin and shRNA MALAT1 in AGS cancer cell line has been
investigated. Also, the toxicity induced by this targeted vector on the growth, migration and death
rate of gastric cancer cells was studied, and the effect of cobalt ferrite nanocomplex on the expression
level of MALAT1 LncRNA related to gastric cancer drug resistance was also investigated
Methodology: Pure cobalt ferrite nanoparticle was made by hydrothermal method. Using a
multi-step process, it was covered with polyethylene-imine and chitosan, carboxylated cis-platinum
drug and MALAT1 shDNA gene were loaded into it. The morphology, structural and magnetic
properties of nanoparticles were investigated by FT-IR, XRD, VSM, EDAX, FESEM and HRTEM
methods. Then, in order to investigate the biological effects of the prepared nanocomposite, MTT,
Scratch, Apoptosis and RT-PCR bioassays were performed
Findings: The results of this research showed that the magnetic nanocomposite carrying the
desired drug and gene is able to enter the nucleus o