The aim of this study was to assess the chronic
effects of a bio-pesticide (NeemAzal) on some biochemical
parameters of grass carp (Ctenopharyngodon
idella) under laboratory conditions. At first,
96 h LC50 of the pesticide was determined using
probit analysis software and was found to be
0.73 mg L
1 of the active ingredient (azadirachtin)
of the pesticide. Fresh fish were then divided
into three treatments and a control and were
exposed to sublethal concentrations (10%, 20%
and 30% of the 96 h LC50 value) of the pesticide
for a month. The biochemical examination of the
blood showed a remarkable decrease in values of
protein, albumin, AST, ALT, ALP and LDH
(P < 0.05).