Background:Today, the use of Spirulina supplements has become popular among resistance athletes, while little information is available about the effects of this supplement and resistance training on various organs of the of the body. Aim: The purpose of this study is to investigate the combined effect of resistance training and spirulina supplementation on EGF/ERK1/2/TSC2/mTOR signaling in kidney male rats.Methodology: The present study was an experimental and laboratory study with a statistical population of 32 young male Sprague-Dawley rats, 3 months old and with a mean weight of 150±20 g, who were randomly divided into four 8-week groups: resistance training, Spirulina supplementation, training + supplementation, and control. The resistance training protocol consisted of eight weeks of climbing a rodent ladder to a height of one meter, performed every other day and three to four repetitions per session. The Spirulina group received 200 mg/kg/day of Spirulina supplementation dissolved in drinking water for 8 weeks. Sampling was performed 24 hours after the last training session.Then, the expression level of the dependent variables of the study was measured using the REAL TIME-PCR method. The variance analysis test was used to analyze the data. Findings: There was no significant increase in EGF factor in any of the groups compared to the control group. Also, compared to the control group, there was a significant increase in ERK factor in the Spirulina supplement group compared to the control group (p=0.03), but there were no significant changes in the other two groups compared to the control. There was no significant effect in TSC2 factor in any of the groups compared to the control group. Compared to the control group, mTOR gene expression was significantly higher in the Spirulina group (p=0.01), in the resistance training group (p=0.16) and the interaction of training and supplementation (p=0.06) despite the increase in gene expression, there were no significant