Seyed Javad Hosseini

Evaluation of the efficiency of insulin-producing cells resulting from adipose tissue mesenchymal stem cell differentiation in glucose sensingand glucose stimulated insulin secretion Objective:Since insulin-producing cells resulting from stem cell differentiation cannot be as effective as pancreatic beta cells in regulating insulin-dependent glucose secretion, it seems necessary to identify the factors influencing this process.Therefore, this study was performed to investigate the changes in the expression of Glut2, ENO1, IGF-IR, Glucagon by insulin-producing cells resulting from the differentiation of adipose-derived mesenchymal stem cells. Methods:Adipose-derived mesenchymal stem cells were differentiated into insulin-producing cells in a 14-day protocol using nicotinamide and ITS.The ability to produce insulin was confirmed by DTZ staining.Insulin and glucagon concentrations were determined by ELISA and the expression of Glut2, ENO1 and IGF-IR genes were measured by real time PCR.Results:DTZ staining confirmed the presence of insulin secreting granules in insulin-producing cells.Differentiated cells secreted significantly more insulin and glucagon compared to undifferentiated cells.The differentiated cells showed significantly higher amounts of Glut2 and IGF-IR gene expressions compared with undifferentiated cells.While the expression of ENO1 was not significantly different between two groups. Conclusion: The obtainedinsulin-producing cells exhibit the ability to naturally absorb glucose, perform glycolysis, and glucose-dependent insulin secretion in vitro. .Keywords: Insulin producing cells, Adipose tissue mesenchymal stem cells, Glut2, ENO1, IGF-IR, Glucagon