Seyed Javad Hosseini

Background: Endoxylanases, officially named endo-1,4-b-xylanase, play an essential role in the production of xylose, which is a primary carbon source for cellular metabolism. The direction of the enzymes market shows that xylanase and cellulase along with pectinases will have a major share in the global enzyme market of about 20%. It is estimated that the world market for xylanase enzymes is more than 200 million dollars. The reason for much attention on xylanase is due to its diverse applications in industrial processes such as improving the digestibility of animal feed, biodyeing of paper pulp, textile industry, production of xylooligosaccharides, improving texture in bread industry products, clarifying juice and wine, the process of peeling tree bark, and converting Lignocellulosic wastes are valuable products such as ethanol, sugar syrup and gas, and liquid fuels. Methodology: To produce the bacterial xylanase enzyme, Pxyn was amplified from the genome of gram-negative Pseudomonas syringae bacteria with high precision by PCR. Then, it was cut with the help of enzymatic digestion and transferred into the pET26b(+) vector, and finally, in a suitable expression host, by induction The lac promoter was expressed, and finally, the expressed enzymes were analyzed by acrylamide gel (SDS-PAGE). Conclusions: The weight of the enzyme sequence extracted from Pseudomonas syringae was estimated to be 41.03 kDa with a length of 370 amino acids. Also, the sequencing results indicate the extraction of endoxylanase enzyme from Pseudomonas syringae bacteria.