Aloe vera has been introduced as a drought tolerant species. However irrigation and nutrition management is necessary to achieve potential quantitative and qualitative yield. In order to investigate biofertilizers effect on Aloe vera quantitative and qualitative yield and water productivity as a medicinal plant under drought stress, a split plot experimental design base on randomized complete blocks with three replications was conducted in 2016-2017 and 2017-2018 cropping seasons in Dashtestan, Boushehr province, Iran. The main-plot included irrigation regimes at four levels: 25%, 50%, 75%, and 100% supply of water requirement (WR) and the sub-plot included biofertilizers at four levels including supplying mycorrhizal fungi (MF) (Glomus mosae), Barvar2 phosphate fertilizer (phosphorus-solubilizing bacteria (PSB) (Pseudomonas putida strain P13 and Pantoea agglomerans strain P5), MF+PSB, and control (without any biofertilizers).