In the current study, the effects of different levels of probiotics, Lactobacillus plantarum in the diet of Asian sea bass, Lates calcarifer on body compositionwere investigated. This experiment was carried out with three treatments and a control group for a period of four weeks. The treatments were included 0, 1×106, 2×106, 3×106 colony forming unit (CFU) gram probiotic in each gram of basic diet. Fish with an average weight of 50.26 ±0.89 grams were randomly distributed in tanks and in each tank stocked 20 pieces of fish. Feeding was done twice a day. The obtained results indicated that the different levels of probiotic did not affect protein, ash and moisture values between the treatments and the control group (P> 0.05). Moreover, fat value in the fish T2 and T3 was significantly higher (P< 0.05) than the fish control and T1. Also, nitrogen free extract (NFE) value varied significantly (P < 0/05) between the control and the fish T3. Overall, this study indicated that this probiotic at the high level can positively influence on body composition of Asian sea bass.