محمود نفیسی بهابادی

The marine fish larvae mostly rely on livefeed, with certain nutritional deficiencies such as poor long chain fatty acids, in spite of recent progress in developing artificial feed. Thus, the present experiment was conducted to evaluate a 30-day dietary effects of enriched rotifer (Brachionus rotundiformis) and Artemia nauplii (Artemia franciscana) with docosahexaenoic acid (DHA) on performance of yellowtail sea bream (Acanthopagrus latus) larvae. For this purpose, 60,000 larvae from wild broodstocks were stocked in 12 tanks (50 larvae/L) supplied with green water (0.5 ×106 cell/ml Nannochloropsis. sp). The rotifer and Artemia were enriched with four experimental emulsions contained four levels of DHA including very low (6 %, DHA-VL), low (12 %, DHA-L), medium (24 %, DHA-M) and high (37.8 %, DHA-H). Larvae were fed with rotifers from day 2–20 post hatch (DPH) and Artemia metanauplii (Instar-II, from 16 to 30 DPH). After the 30-day dietary trial, survival rate and growth performance of larvae were found not affected by different levels of DHA (P > 0.05). The activity of digestive enzymes including total proteases, lipase, amylase, alkaline phosphatase, and leucine-alanine peptidase showed some fluctuations among the experimental groups. However, in the most of the sampling intervals, except for protease activity, the activity of digestive enzymes did not change significantly among the experimental groups. The n–3 polyunsaturated fatty acids, particularly eicosapentaenoic acid (EPA) and DHA, were the highest in the whole body of larvae from DHA-M treatment. The DHA/EPA ratio in the whole body of larvae was significantly increased with increasing DHA level (P < 0.05). Based on our findings, very low levels of DHA is enough for supporting normal growth, survival and metamorphosis in developing A. latus larvae up to 30 days post hatching.