ماندانا زارعی

The aim of present study was to evaluate the ability of the n-hexane, dichloromethane, ethyl acetate, ethanol and water extracts algae brown Cystoseira trinodis to neutralize leathaly, edematogenic, hemolytic, coagulant and proteolytic activities of Ecish carinatos venom. After extraction and evaporation of solvents, in order to determine the most suitable concentration of venom for each test, different concentrations of it were prepared. Three concentrations 120,240,360 mg/ml have been used for the investigation of coagulant activity, three concentrations 90,180, 270 ?g/ml to anti-hemolytic activity and three concentrations50,100,150 ?g/ml to antiedema activity, four concentrations 40,160,400,800 ?g/ml to the anti-proteolytic activity, and one concentration ?g/ml for the venom lethality. The results showed that the antihemolysis activity of hexane extract with concentration 270 ?g/ml and dicloromethane extract with concentration of 90 ?g/ml in comparison with the control group had inhibitory effect and this inhibitory was significant. In anti-proteolytic activity, ethyl acetate extract with concentration 400?g/ml showed an inhibitory effect. In the study of the anticoagulation activity of the venom, the dichloromethane extract with the concentrations of 120,240,360 mg/ml, ethyl acetate extract with the concentration 1200,2400 ?g/ml, ethanol extract with the concentration of 120 mg/ml, water extract with the concentration of 120,240,360 mg/ml proved effective on this activity and Significant differences were observed with the control group. In the evaluate of the activity of edema, ethanol and dichloromethane extract with the concentration of 150 ?g/ml proved effective and Significant differences were observed with the control group. Through the analysis of the effect of extracts on lethality at mouse, all extracts delayed the death time, but the most effective extract was dichloromethane and the mice were survived to three hours after injection of venom. Significan