Background: The alginate lyase enzyme extracted from the native bacteria of the Persian Gulf is less dangerous than the enzymes extracted from pathogenic bacteria.
Aim: Extraction and relative purification of alginate lyase enzyme from a native bacterium of the Persian Gulf.
Methodology: In this study, the enzyme alginate lyase was extracted from the indigenous bacteria of the Persian Gulf and some of its properties were determined. After polymerase chain reaction and sequencing, three strains capable of producing alginate lyase enzymes were isolated and identified. In this study, the alginate lyase enzyme was partially purified using an ammonium sulfate precipitation and a dialysis bag, and the molecular weight of the enzyme was determined by SDS-PAGE electrophoresis. The protein concentration of the alginate lyase enzyme produced in this study was determined using the Bradford method and the enzyme activity was evaluated in the presence of various chemical compounds and ions.
Findings: In this study, after performing PCR and sequencing, three strains of Pseudomonas koreensis strain JCR-18, Lelliottia amnigena strain NCTC12124, and Bacterium fjat-scr-2 with the ability to produce alginate lyase enzyme were isolated and identified Among them, Pseudomonas koreensis strain JCR_18 was selected for further investigations and studies as it showed the highest amount of alginate lyase enzyme production in liquid medium. Its molecular weight was about 60 kilodaltons. The maximum activity of the enzyme was achieved at a pHof 7 and a temperature of 30°C and the enzyme was stable in the pH range (5-9-5.5) for 90 minutes. The protein concentration of the alginate lyase enzyme produced in this study using the Bradford method was 0.88 mg/ml. Magnesium and calcium ions stimulate the enzyme activity of alginate lyase, while aluminum and iron can inhibit the enzyme activity. Furthermore, the enzyme activity was examined in the presence of chemical compounds, and the results showed t