Disulfide oil is one of the by-products of oil and gas refining units which is produced in the process of removing mercaptans and sweetening light hydrocarbons. Deposition of elemental sulfur and asphaltene is one of the constant challenges of the oil and gas industry, and so far, various methods have been used to prevent or remove these deposits. The use of inhibitors and dispersants is one of the common methods used by the industry to prevent or treat these types of sediments. The main goal of this research is to investigate and evaluate the feasibility of using DSO as an additive to inhibit the precipitation of elemental sulfur and asphaltene. In this research, physical and chemical properties of DSO of Fajr Jam Gas Refinery such as density, viscosity, pH, distillation parameters and Reid Vapor Pressure (RVP) have been measured by ASTM standard methods. Then, DSO was oxidized at different times with an active oxygen production device by plasma method. Solubility of elemental sulfur was done in DSO, organic solvents, petroleum products and their combination. The type and effective amount of solvent for elemental sulfur were determined from the design of the experiment using the Taguchi and the weight difference methods in the solubility value. Corrosion of copper and carbon-steel coupons were investigated to determine the quality, quantity, and corrosion rate in disulfide oil mediums before and after oxidation. Synthetic oil with different concentrations of asphaltene was prepared according to the standard method and scientific texts. Ultraviolet spectrophotometry method was used to determine the onset point of asphaltene precipitation of synthetic and crude oil and also to measure the amount of asphaltene. IP143 standard method was used to determine the amount of asphaltene in the crude oil sample of this study. The additive effect of pure DSO, oxidized DSO with different times, toluene and 1-butanol in preventing precipitation and asphaltene content of petroleum