Objectives: The recognition of repressive elements and functional nuclear factor binding sites or transcription factor binding sites (TFBSs) is the first step on the way to deciphering the DNA regulatory code. For both, immune systems and poultry breeding programs, study the expression and identification the TFBSs in promoter regions of defense gene are very important. We here performed an In Silico study to recognize binding sites of transcription factors in the upstream region of Gal2 gene in Khozestan local chicken, which essential for the immune system. Materials