06 آبان 1400

غلامرضا عبدی

مرتبه علمی:
نشانی: پژوهشکده خلیج فارس - گروه زیست فناوری
تحصیلات: بیوتکنولوژی گیاهان دارویی
تلفن:
دانشکده: پژوهشکده خلیج فارس

مشخصات پژوهش

عنوان
بررسی درون شیشه ای تنحمل شوری و خشکی بدو توده شنبلیله ایرانی Trigonella foenum– graecum
نوع پژوهش پارسا
کلیدواژه‌ها
Callus induction, Drought Stress, Salinity Stress, Fenugreek, Trigonelin
پژوهشگران افسانه توانگر (دانشجو) ، لیلا کرمی (استاد راهنما) ، محمد هدایت (استاد راهنما) ، غلامرضا عبدی (استاد مشاور)

چکیده

In this study, micropropagation of two Iranian Fenugreek populations (Ardestan and Borazjan) and their morphological and biochemical responses to salinity and drought stresses in in vitro culture condition were conducted using factorial experiment in a completely randomized design in three replications. Different explant type (terminal bud, cotyledon and epicotyledon) were cultured in MS medium contain different concentration of plant growth regulators such as Kin (0, 0.5 and 1 mg / l) and 2,4-D (0.5, 1 and 2 mg / l). Murashige and Skoog (MS) medium supplemented with 1 mg/l kinetin and 2 mg/l 2,4-D showed the highest callus proliferation rate per explants in both papulation. The highest amount of callus volume was obtained from the explants of the terminal bud. Proliferated calli from terminal bud explant were green and yellowish, from cotyledon were yellowish to white with soft texture, and the cotyledons were greenish and compact. The results of salinity stresses with different concentrations of sodium chloride (0, 70 and 120 mM) and drought stress with polyethylene glycol (0, 5 and 10%) showed that both stresses decreased callus growth and increased total protein, proline, catalase, peroxidase and Trigonelin content in both papulation. Trigonelin measurement showed that Brazjan papulation had higher Trigonelin content in vitro than Ardestan Papulation.