|
Abstract
|
In order to investigate the genetic diversity in twenty varieties of canola, nine primers of ISSR markers were used. Eight ISSR primers were created the most polymorphic from total primers. Ninety nine bands were produced from 9 ISSR which 79 of them were polymorphic. In this study, the average band number per primer and variety was 11 and 4.95 amplified, respectively. The number of polymorphic bands ranged from 0 to 14. The average number of polymorphic bands per primer and variety were 8.77 and 3.59, respectively .The highest Polymorphic Information Content (PIC) calculated for MM6 primer (0.78). The (Hb10 primer) (10.47) was accounted the highest index (MI), as well. Tree diagram of Cluster analysis using NTSYS software and UPGMA method based on Simple mathing similarity matrix divided varieties into 6 clusters. Furthermore, a Two-dimensional plot of the Principal Coordinate Analysis (PCoA) was drawn and the results were compared with cluster analysis. Varieties grouping based on two methods had a great similarity. In this study, the similarity between dendrogram and the Two-dimensional indicated the existence of diversity in the germplasm studied.
The greatest genetic similarity existed between Elite and Rinbow varieties. The two spring varieties, Hysyn 110 and Delgan, were located far apart each other, genetically. These varieties can be used as crossing parents in plant breeding programs with high heterosis. The genetic pattern did not conform to the geographic patterns. High band production (99) and polymorphism (average 79%) in studied cultivars indicated the ISSR markers ability to grouping different canola varieties. In this study, the primers HB10, MM6 and HB14 had the highest PIC and MI and also represented very high polymorphism (100%). So, these primers were introduced as useful and efficient primers in the present study. The results of this research demonstrated the reliability of ISSR markers to detect a high level of polymorphism between canola vari
|