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Abstract
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In this study, the effect of different dietary nucleotide levels (0, 0.2, 0.4 and 0.6%) was investigated on growth indices and some haemolymph biochemical parameters of Litopenaeus vannamei female broodstock during 30 days. broodstocks were fed as co-feeding (2 times with fresh food and 2 times with concentrate feed). after 3 weeks of feeding, eyestalk of broodstocks was ablated with hot scissors. hemolymph was sampled in three times, at the first day of the trial, 21 days (before eyestalk ablation) and 30 days (9 days after eyestalk ablation) after starting the feeding trial. In experimental period, water parameters including temperature, dissolve oxygen, salinity and pH recorded daily as 27.79 ± 1.24 ?C, 5.78 ± 0.41 ppm, 31.82 ± 3.1 ppt and 7.9 ± 0.18, respectively. haemolymph biochemical parameters of female broodstock including glucose, total cholesterol, triglyceride, calcium, albumin, total soluble proteins, high density lipoprotein and low density lipoprotein measured in three phases (start of feeding, 21 [pre eyestalk ablation period] and 30 days after start feeding) by biochemical standard methods. the results showed that body weight increase, specific growth rate and daily growth rate in samples treated by dietary nucleotide showed no significant difference (p > 0.05) compared to control group. analysis of biochemical heamolymph showed that the glucose, total cholesterol, triglyceride, albumin, total protein were significant increasing (p < 0.05), in all groups compare to control group. In conclusion 0.4 % nucleotide can improve the dietary sexual maturation of Litopenaeus vannamei.
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