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Abstract
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Green tiger shrimp (Penaeus semisulcatus) is one of the most important members of the Penaeida family in the Persian Gulf. Two different morphological groups of this species live in the Persian Gulf, The most important difference between them is antenna color pattern. Group with Striped antenna (MGI) is considered as a species and those with a uniform antenna (MGII) as a subspecies. geometric morphology and mitochondrial markers of ND2 and COI were used to compairing these two. study morphology, the photograph was taken from the carapace and the images were analyzed with ImageJ, MorphJ and PAST softwares. Genomic DNA extracted from the shirmps muscle was used as template to amplify ND2 and CO1 genes. Part of the two genes was sequenced and translated using the Expasy software. The nucleotide sequences and amino acids of these samples were analyzed with ClustalW, MEGA7 and DNASP v6 software. The results of morphological studies, which were obtained by comparing the mean carapace shape of the two groups, showed that carapace in both groups is very similar and The only difference is the more width carapase in MGI than MGII. Molecular results showed that the genetic distance between MGI and MGII was 609 pairs of ND2, 21% to 27% and 605 pairs of COI, 16% to19%. Based on both gene, the genetic differentiation between the two groups is very high but the genetic flow is very low ,and almost at zero, which indicates a complete separation of production of two groups. Overall, intra-group genetic variation in MGI was more than MGII. The results showed that ND2 in comparison with COI shows a higher level of fescribed as a marker for the evaluation a species populations.
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