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Abstract
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new and simple strategy is applied to resolve kinetic profile for the reaction of an analyte
in unknown matrices, using standard addition method (SAM). The proposed method uses
kinetic spectrophotometric data obtained by standard addition of analyte into unknown mixtures
followed by the reaction of analyte with a proper reagent. The proposed method extracts kinetic
profile for the reaction of an analyte by averaging the kinetic profiles obtained by subtraction of
kinetic profiles after and before standard addition. The rate constant can be obtained using computational
curve fitting. The performance of method was evaluated by using synthetic data as well as
several experimental data sets. The proposed method can be applied to obtain kinetic profiles of the
reactions in the presence of additive interference as well as multiplicative interferences. Hydroxylation
reaction of diphenylcarbazide (DPCI) in the presence of diphenylcarbazone (DPCO) as a real
system at various pHs was also studied by the present method. The rate constant and the order of
the hydroxylation reaction were determined from extracted kinetic profiles
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