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Abstract
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Chitinase production by newly isolated Serratia
marcescens B4A was optimized following Taguchi’s
array methods. Twenty-three bacterial isolates were
screened from shrimp culture ponds in the South of
Iran. A chitinase-producing bacterium was isolated
based on it’s ability to utilize chitin as the sole carbon
source. The isolate designated as B4A, was identified
as Serratia marcescens based on its 16S rRNA
sequence and key morphological, physiological and
biochemical characteristics. The cultivation of Serratia
marcescens B4A in the appropriate liquid medium
resulted in production of high levels of chitinase. The
malt extract and colloidal chitin represented the best
nitrogen and carbon sources, respectively. Chitinase
production by Serratia marcescens B4A was optimized
following the Taguchi orthogonal array (OA) for the
design of experiments (DOE). Statistical experimental
design via the Taguchi method was applied to determine
the optimal levels of physical parameters and key
media components in the medium, such as temperature,
pH, NaCl and chitin concentrations. The results of
this study showed that temperature of 30?C, pH 7.9,
NaCl 0.1% (w/v) and chitin 1% (w/v) are optimal conditions
for this protocol.
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