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Abstract
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In Bushehr province of Iran, Avicennia
marina trees have grown in Bordekhoon (Mond
Protected Area) and Assaluyeh (Marine National Park
of Nayband). Contrary to Bordekhoon, Assaluyeh is aAssaluyeh pollen extracts, respectively, and mice
serum samples were used for immunoblotting. Pollen
characteristics were studied using light and scanning
electron microscopes. SDS-PAGE showed some differences
between pollen protein pro?les of two
regions. Immunoblotting assay detected that pollens
have two allergenic bands and the protein band at
100 KD is the common allergenic protein in two
regions. Light microscopy revealed that the development
of anther wall was basic type and some abnormalities
were observed in microspores and pollens of
Assaluyeh. Scanning electron microscopy studies
showed that the apertures in considerable numbers of
Assaluyeh pollens were closed. The comparison of
elemental composition of pollen tectum between two
regions showed that pollens of Assaluyeh have accu-
mulated Cu on their tectum. Results obtained indicated
that environmental pollution can affect protein pro?le,
allergenic bands, structure and elemental composition
of tectum of A. marina pollens.
petrochemical region with environmental pollution.
This study was aimed to studying protein pro?les,
allergenic bands, ontogeny, structure and elemental
composition of tectum of A. marina pollens in
Assaluyeh and Bordekhoon. Pollens were collected
from two regions and extracted in PBS, and protein
pro?les of pollens were determined by sulfate–polyacrylamide
gel electrophoresis (SDS-PAGE). As an
experimental model, 20 female 6–8-week-old Balb/C
mice were divided into two groups. The mice of ?rst
and second groups were sensitized by Bordekhoon and
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