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Abstract
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In this paper, the electrochemical method was used as a rapid and sensitive method to measure the antioxidant
activity of Acanthophora red macro-algae (collected from the Persian Gulf coast of Bushehr-Iran). The algae
extract was obtained by a combination of maceration and microwave methods, and then the important volatile
and bioactive compounds were identified by GC-Mass analysis. To prepare the sensor and improve the electrochemical
signal, gold nanoparticles synthesized by the green method were used to modify the electrode
surface. Differential pulse voltammetry method (DPV) was used to investigate the inhibitory effect of the extract
on ABTS and DPPH radicals.
To investigate the ability of the Acanthophora algae extract to prevent DNA damage induced by Fenton
reaction, an electrochemical biosensor was designed based on the immobilization of human interleukin-2 (IL-2)
gene probe on the surface of gold nanoparticle-modified carbon screen-printed electrode. Then, the protective
effect of the algae extract was investigated by electrochemical impedance spectroscopy. Finally, the toxicity
effect of Acanthophora algae extract on the HT-29, SKOV3, MCF-7, and SKMES-1 cancer cell lines was evaluated
by MTT assay.
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