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Abstract
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This study was conducted to assess the evaluate genetic and morphological differentiation of Sparidae SP. in Bushehr province. Morphometric measurements and meristic counts were calculated for five species of family Sparidae. Differences in body shape between species using geometric morphometric techniques and digital photography and to put landmarks on two-dimensional images were. Obtained data were analysed by multivariate principal component analysis (PCA) and canonical variate analysis (CVA), and discriminant function analysis (DFA). The results showed significant differences between the body shapes of studied species (P<0.05). Morphometric shape analysis showed that the main factor in the separation of these species are the size of the head. In order to study the phylogenetic relations of sparidae family, COI sequencing methodology was used. Haplotype diversity was caclculated 0.500 for Acanthopagrus latus, Acanthopagrus bifasciatus 1, Argyrops spinifer 1, Sparidentex hasta 0, and Crenidens crenidens indicus was 0.833. The highest Haplotype diversity was in Argyrops spinifer and Acanthopagrus latus and the lowest was in Sparidentex hasta species.
Phylogeny trees drived from COI gene sequencing using Neighbor Joining and Maximum likelihood trees showed significant differentiation of the samples. results indicate that the the species of a genus that highly apparent similarity are put in one branch. COI sequences obtained in this study was phylogeneticaly compared to other Sparidae sequences obtained from gene bank were. In the present study, the average GC in Acanthopagrus latus, Acanthopagrus bifasciatus, Argyrops spinifer, Sparidentex hasta and Crenidens crenidens indicus were 44, 43.1, 47.1, 46.1 and 46.8 respectively. Based on the results of the present study, apparently Argyrops spinifer compared to other species of Sparidae in the waters of the Busher province is more ancient. The genetic distances between samples was calculated between 0.07 - 0.21, which i
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