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Abstract
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The present study evaluated the antimutagenic and anticancer activities of A. marina leaf extract. The
antimutagenic activity of ethanol and water extracts of A. marina leaf (concentration 10 mg/plate for 48 h) was evaluated
using the Salmonella typhimurium TA100 strain in the Ames test with ( S9) and without (S9) metabolic activation, and
its anticancer effect with concentrations of 200, 400, 450, 500, 600, and 800 µg/ml of ethanol extract for 24, 48, and 72 h on
human leukaemic cell line HL-60 was assessed by MTT assay. The ethanol and water extracts of A. marina were revealed
to have antimutagenic activity in the Salmonella mutation assay. The number of mutant colonies decreased in the presence
of ethanol and water extract with ( S9) and without (S9) metabolic activation. The ethanol extract showed a higher
antimutagenic effect than the water extract, with an inhibition rate of 71% on the mutated bacterium. The MTT cell viability
test showed an induction of cytotoxicity in HL-60 cells treated with the extract with IC
values of 600, 400, and 280 µg/ml
after 24, 48, and 72 h, respectively, in a concentration and time-dependent manner. Flow cytometric analysis con?rmed
the induction of apoptosis in the cells. Collectively, these results suggest that the Avicennia marina leaf extract contains
bioactive compounds with antimutagenic and antileukaemic effects.
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