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Abstract
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Silver nanoparticles are the most important nanomaterials for antibacterial uses
and are famous for their strong inhibitory and antibacterial effects. In recent years,
extensive studies have been undertaken on the use of antimicrobial properties of silver,
incorporated within aquaculture industry. To evaluate the scientific basis for the use of the
nano silver in shrimp aquaculture, in this study the antimicrobial activities of colloidal
nano silver with two different sizes (16.62 and 22.22 nm) was evaluated against gram
negative bacteria, V. harveyi. Before the experiments, cAgNPs were characterized using
several analytical techniques. Well diffusion method, micro-dilution tests (MIC and MBC)
and kinetic of death were used to evaluate the bactericidal activity of the nanoparticles.
Results showed that MIC and MBC values of cAgNPs in both studied sizes are equal
(MIC = MBC). Best bactericidal kinetics in the presence of 16.62 and 22.22 nm
nanoparticles obtained at 4 and 6 h, respectively. The obtained results suggested that
smaller silver nanoparticles had a faster antibacterial activity than the larger particles.
According to the obtained results, the activity of cAgNPs against V. harveyi is fast and has
potential for the treatment of bacterial infection in aquaculture
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