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Abstract
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A 60-day feeding trial was performed to assess the dietary effect of Lactobacillus casei as a probiotic supplement on some
serum biochemical parameters and liver histopathology in common carp fry after exposure to iron oxide nanoparticles
(IoNPs). Six treatments were prepared as follows: control (no IoNP exposure and no dietary probiotic), P6: 106
CFU/g probiotic
diet, P7: 107
CFU/g probiotic diet, NPs: 0.15 mg/l IoNPs, NPs + P6: 0.15 mg/l IoNPs with 106
CFU/g probiotic diet,
and NPs + P7: 0.15 mg/l IoNPs with 107
CFU/gprobiotic diet. Based on the results, serum aspartate aminotransferase and
alanine aminotransferase levels were significantly increased in 0.15 mg/l IoNPs, P7, and NPs + P6 treatments compared to
the control group. In addition, the examination of antioxidant enzymes showed a significant increase in the levels of cortisol
and glutathione S-transferase as well as malondialdehyde level. IoNPs also caused significant histopathological changes in
the fish liver during the experiment such as hyperemia in sinusoidal spaces, hepatocytes vacuolation and necrosis, pyknosis,
and disruption of hepatic lobules and atrophy. Results revealed the protective effects of dietary L. casei to mitigate the adverse
impacts of IoNPs on the physiological processes of common carp.
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