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Abstract
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The main objective of this study was to investigate the polymorphism of GDF9 and
BMPR1B genes and their relationship with litter size in Markhoz goats. The polymorphism
of GDF9 and BMPR1B genes as well-documented genes regarding fecundity
in sheep and goat was investigated using RFLP-PCR and a tetra-primer amplification
refractory mutation system-PCR (T-ARMS-PCR) in Markhoz goats. The 164 blood
samples were collected from the raised goats in Sanandaj Markhoz goat Performance
Testing Station. The DNA extraction was carried out by salting-out procedure, and
then, PCR was performed using four and two pairs of primers to detect polymorphism
in GDF9 and BMPR1B genes, respectively. To disclose GDF9 loci polymorphism,
PCR products were digested with SspI (G3288A), PvuII (G423A), MvaI (A959C)
and MspI (G1189A) restriction enzymes. The results showed that these mutations are
available in tested animals. Parity had no significant effect on litter size. Also, the effects
of different genotypes of GDF9 and BMPR1B had no significant effect on litter
size. Further studies with a high number of animals with minimum relatedness for
testing the association of these SNPs and others in the fecundity genes with reproductive
traits may be worthwhile.
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