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Abstract
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Low molecular weight Polycyclic Aromatic Hydrocarbons (PAHs) are widely concerned
due to their significant health risks such as carcinogenicity, reproductive- and
endocrine-disrupting effects, immunotoxicity and neurotoxicity on human and marine
animals. The induction pattern of xenobiotic metabolizing cytochrome P4501A
(CYP1A1) gene was studied on 150 spotted scat Scatophagus argus (weight 32±3 g)
captured from Northern Persian Gulf and exposed to 0, 5, 10, 15, 20 mg kg-1 of
benzo[a]pyrene in 100L aquarium. The induction of CYP1A1 was quantified using
reverse-transcription real-time PCR from treated fish liver samples. The results
revealed that the expression of CYP1A1 gene in S. argus liver is time-dependent and
directly correlated to the concentration of B[a] P, therefore the study provides the
basis for further use of CYP1A1 of this commercially ad ecologically important species
as a biomarker.
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