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Abstract
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Background: The current work investigated the immunological
features of insulin-producing cells (IPCs) generated from rat
adipose-derived mesenchymal stem cells (ADSCs) both in vitro
and in vivo.
Methods: The research was carried out at Ahvaz Jundishapur
University of Medical Sciences in 2023. ADSCs were derived
from rat adipose tissues and differentiated into IPCs. The
control group included undifferentiated ADSCs. The amount
of secreted insulin was measured using ELISA. The expression
of major histocompatibility complex-I (MHC-I) and MHC-II,
cluster of differentiation 40 (CD40), and CD80 by IPCs in vitro
was assessed using Western Blot analysis. The in vivo study
was performed on 10 male diabetic rats. The experimental
group received 107
IPCs in the peritoneal cavity. The control
group received 107 undifferentiated ADSCs. After 4 hours, the
expression of CD3a and CD45 by immune cells collected from
the peritoneal cavity was measured using flow cytometry. All
parameters were statistically analyzed using a t test.
Results: The differentiated cells secreted much higher amounts
of insulin than the control group (P=0.04). IPCs exhibited higher
expression of MHC-I and MHC-II, CD40, and CD80 (P=0.02,
P=0.008, P=0.07, and P=0.02, respectively). The experimental
group showed higher levels of CD3a and CD45 expression than
the control group (P=0.07, P=0.04, respectively).
Conclusion:Functional IPCs generated by ADSCs differentiation
exhibited immunogenic activity both in vitro and in vivo.
Immune-modulating strategies are required for the effective
transplantation of the differentiated IPCs generated in our study.
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